Analysis Note

ControlMouse brain nuclear extract

Application

Research CategoryNeuroscience

Research Sub CategoryDevelopmental Signaling

Anti-NeuN (rabbit) Antibody, Cat. No. ABN78, is a highly specific rabbit polyclonal antibody that targets NeuN and has been tested in Immunocytochemistry, Immunofluorescence, Immunohistochemistry, and Western Blotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

RNA binding protein fox-1 homolog 3 (UniProt Q8BIF2; also known as Fox-1 homolog C, Fox-3, Hexaribonucleotide-binding protein 3, NeuN, Neuronal nuclei) is encoded by the Rbfox3 (also known as D11Bwg0517e, Hrnbp3) gene (Gene ID 52897) in murine species. The RNA-binding Fox (Rbfox) family of splicing factors is comprised of three members, Rbfox1 (Fox-1 or A2BP1), Rbfox2 (Fox-2 or RBM9), and Rbfox3 (Fox-3, HRNBP3 or NeuN). Rbfox proteins regulate splicing of many neuronal transcripts (pre-mRNAs) by binding the sequence (U)GCAUG in introns flanking alternative exons. A (U)GCAUG motif that lies downstream of the alternative exon generally promotes Rbfox-dependent exon inclusion, whereas an upstream motif will usually repress exon inclusion. Originally characterized as a marker of post-mitotic neurons and named neuronal nuclei (NeuN), Rbfox3 plays a role in promoting neuronal differentiation through alternative splicing of Numb pre-mRNA during brain development. Rbfox3-knockout in mice is reported to result in defective hippocampal gene expression, defects in synaptic transmission and plasticity in the dentate gyrus, as well as increased seizure susceptibility and decreased anxiety-related behaviors. Likewise, human RBFOX3 gene mutations have been linked to neurodevelopmental delay, cognitive impairments, autistic features, and epilepsy.

Immunogen

Epitope: N-terminus

GST-tagged recombinant mouse NeuN N-terrminal fragment.

Legal Information

ALEXA FLUOR is a registered trademark of Life Technologies

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Affinity purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in mouse brain nuclear extract.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected NeuN in 10 µg of mouse brain nuclear extract.

Specificity

This rabbit polyclonal antibody targets NeuN N-terminal region that ends at the start of RNA recognition motif (RRM). Target region is present in all human, mouse, and rat spliced isoforms reported by UniProt (A6NFN3, Q8BIF2, D4A2H6, A0A0G2K2J4).

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~48/42 kDa observed. 33.87/35.12 kDa (human isoform 1/2), 40.61/39.05/35.64/34.07/35.63 kDa (mouse isoform 1/2/3/4/5), 40.59kDa (rat; UniProt D4A2H6), 34.05 kDa (rat; UniProt A0A0G2K2J4) calculated. Uncharacterized bands may be observed in some lysate(s).